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Chemically sulfated Escherichia coli K5 polysaccharide derivatives as extracellular HIV‐1 Tat protein antagonists
Author(s) -
Urbinati Chiara,
Bugatti Antonella,
Oreste Pasqua,
Zoppetti Giorgio,
Waltenberger Johannes,
Mitola Stefania,
Ribatti Domenico,
Presta Marco,
Rusnati Marco
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.05.033
Subject(s) - sulfation , extracellular , internalization , transactivation , heparan sulfate , chemistry , biochemistry , receptor , biology , cell , transcription factor , gene
The HIV‐1 transactivating factor (Tat) acts as an extracellular cytokine on target cells, including endothelium. Here, we report about the Tat‐antagonist capacity of chemically sulfated derivatives of the Escherichia coli K5 polysaccharide. O ‐sulfated K5 with high sulfation degree (K5‐OS(H)) and N , O ‐sulfated K5 with high (K5‐N,OS(H)) or low (K5‐N,OS(L)) sulfation degree, but not unmodified K5, N ‐sulfated K5, and O ‐sulfated K5 with low sulfation degree, bind to Tat preventing its interaction with cell surface heparan sulfate proteoglycans, cell internalization, and consequent HIV‐LTR‐transactivation. Also, K5‐OS(H) and K5‐N,OS(H) prevent the interaction of Tat to the vascular endothelial growth factor receptor‐2 on endothelial cell (EC) surface. Finally, K5‐OS(H) inhibits α v β 3 integrin/Tat interaction and EC adhesion to immobilized Tat. Consequently, K5‐OS(H) and K5‐N,OS(H) inhibit the angiogenic activity of Tat in vivo. In conclusion, K5 derivatives with distinct sulfation patterns bind extracellular Tat and modulate its interaction with cell surface receptors and affect its biological activities. These findings provide the basis for the design of novel extracellular Tat antagonists with possible implications in anti‐AIDS therapies.