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Stabilization of flagellar filaments by HAP2 capping
Author(s) -
Diószeghy Zoltán,
Závodszky Péter,
Namba Keiichi,
Vonderviszt Ferenc
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.05.029
Subject(s) - biophysics , flagellum , chemistry , microbiology and biotechnology , biology , biochemistry , gene
In vivo growth of bacterial flagellar filaments by self‐assembly of flagellin is promoted by a capping structure composed of a pentameric assembly of hook associated protein 2 (HAP2). Isolated native filaments with intact HAP2 cap exhibited higher melting temperature (Δ T m =4 °C) and significantly increased resistance against heat‐induced depolymerization than non‐capped ones. Reconstituted filaments were also stabilized by HAP2 binding, but the obtained filament–HAP2 complexes were less stable than native assemblies. Their fast depolymerization at elevated temperatures and sensitivity to proteolysis indicated that native‐like filament–HAP2 complexes are rarely obtained by in vitro reconstitution. A procedure was developed to isolate perfectly capped native filaments to facilitate high‐resolution structural analysis.