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CCL3, acting via the chemokine receptor CCR5, leads to independent activation of Janus kinase 2 (JAK2) and G i proteins
Author(s) -
Mueller Anja,
Strange Philip G
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.04.100
Subject(s) - heterotrimeric g protein , janus kinase 2 , janus kinase , stat protein , microbiology and biotechnology , chemistry , c c chemokine receptor type 6 , g protein , chemokine receptor ccr5 , chemokine receptor , phosphorylation , signal transduction , chemokine , biology , receptor , stat3 , biochemistry
The interaction of the chemokine receptor, CCR5, expressed in recombinant cells, with different G proteins was investigated and CCR5 was found to interact with G i , G o and G q species. Interaction with G i leads to G protein activation, whereas G q does not seem to be activated. Additionally, CCR5 activation also leads to phosphorylation of Janus kinase 2 (JAK2). Activation of JAK2 is independent of G i or G q activation. G i protein activation was not prevented by inhibition of JAK, showing that heterotrimeric G protein activation and activation of the JAK/signal transducer and activator of transcription (STAT) pathway are independent of each other.