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Organization of telomeric nucleosomes: atomic force microscopy imaging and theoretical modeling
Author(s) -
Mechelli Rosella,
Anselmi Claudio,
Cacchione Stefano,
De Santis Pasquale,
Savino Maria
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.04.032
Subject(s) - nucleosome , chromatin , dna , atomic force microscopy , biophysics , telomere , chemistry , biology , physics , nanotechnology , materials science , genetics
Telomeric chromatin has peculiar features with respect to bulk chromatin, which are not fully clarified to date. Nucleosomal arrays, reconstituted on fragments of human telomeric DNA and on tandemly repeated tetramers of 5S rDNA, have been investigated at single‐molecule level by atomic force microscopy and Monte Carlo simulations. A satisfactory correlation emerges between experimental and theoretical internucleosomal distance distributions. However, in the case of telomeric nucleosomal arrays containing two nucleosomes, we found significant differences. Our results show that sequence features of DNA are significant in the basic chromatin organization, but are not the only determinant.