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Yap8p activation in Saccharomyces cerevisiae under arsenic conditions
Author(s) -
Menezes Regina A.,
Amaral Catarina,
Delaunay Agnès,
Toledano Michel,
Rodrigues-Pousada Claudina
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.04.019
Subject(s) - saccharomyces cerevisiae , transactivation , arsenic , microbiology and biotechnology , chemistry , nuclear transport , nuclear export signal , function (biology) , biochemistry , yeast , biology , gene , transcription factor , cell nucleus , organic chemistry
Yap8p, a member of the Saccharomyces cerevisiae Yap family, is activated in response to arsenic. Both the mechanisms by which this activation takes place and its regulation have not yet been identified. In this report, we show that Yap8p is not activated at the transcriptional level but, rather, its nuclear transport is actively regulated and dependent on the exportin chromosome region maintenance protein. In addition, it is shown that Cys 132 , Cys 137 and Cys 274 are essential for Yap8p localization and transactivation function both of which are required for its biological activity.