Premium
Cationic oligopeptide‐mediated delivery of dsRNA for post‐transcriptional gene silencing in plant cells
Author(s) -
Unnamalai Narayanan,
Kang Bong Gu,
Lee Woo Sung
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.04.018
Subject(s) - rna silencing , gene silencing , rna , biology , rna induced silencing complex , rnase p , gene , gene expression , oligopeptide , microbiology and biotechnology , small interfering rna , rna interference , messenger rna , genetics , biochemistry , peptide
We have used cationic oligopeptide polyarginine‐12mer (POA) to deliver double‐stranded RNA (dsRNA), prepared in vitro, to tobacco ( Nicotiana tabacum ) suspension cells. POA interacts electrostatically with dsRNA to form a complex. When dsRNA for the GUS or NPTII gene was delivered into cells carrying the same genes, the corresponding mRNA was degraded. Using RNase protection assay we were able to detect 21‐bp small interfering RNA in dsRNA/POA‐treated cells. These results demonstrate that POA can be used to deliver dsRNA to induce post‐transcriptional gene silencing in plant cells.