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Co‐localization of CD3 and prion protein in Jurkat lymphocytes after hypothermal stimulation
Author(s) -
Wurm Susanne,
Paar Christian,
Sonnleitner Alois,
Sonnleitner Max,
Höglinger Otmar,
Romanin Christoph,
Wechselberger Christian
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.03.114
Subject(s) - jurkat cells , stimulation , chemistry , microbiology and biotechnology , cd3 , prion protein , virology , biology , t cell , neuroscience , medicine , immunology , antigen , immune system , cd8 , disease
While long‐term effects of temperature treatment in respect of, e.g., gene‐expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short‐term hypothermal shifts. In this report, we characterized the effects of such a stimulation using the human lymphocyte cell line Jurkat E6.1 and present evidence that warming from 4 to 37 °C for only 2 min is sufficient to cause co‐localization of CD3, prion protein and the lipid‐raft ganglioside GM1 paralleling lymphocyte activation as observed by Ca 2+ mobilization and mitogen‐activated protein kinase‐phosphorylation.