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Characterization and promoter analysis of the mouse nestin gene
Author(s) -
Cheng Leping,
Jin Zhigang,
Liu Li,
Yan Ye,
Li Tingting,
Zhu Xueliang,
Jing Naihe
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.03.097
Subject(s) - gene , microbiology and biotechnology , nestin , characterization (materials science) , chemistry , genetics , computational biology , biology , nanotechnology , materials science , neural stem cell , stem cell
The intermediate filament protein nestin is expressed in the neural stem cells of the developing central nervous system (CNS). Promoter analysis revealed that the minimal promoter of the mouse nestin gene resides in the region −11 to +183 of the 5 ′ ‐non‐coding and upstream flanking region, and that two adjacent Sp1‐binding sites are necessary for promoter activity. Electrophoretic mobility‐shift assays (EMSA) and supershift assays showed that Sp1 and Sp3 proteins selectively bind to the upstream Sp1 site. These results demonstrate an important functionality of Sp1 and Sp3 in regulating the expression of the mouse nestin gene.