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The effect of phalloidin and jasplakinolide on the flexibility and thermal stability of actin filaments
Author(s) -
Visegrády Balázs,
Lőrinczy Dénes,
Hild Gábor,
Somogyi Béla,
Nyitrai Miklós
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.03.096
Subject(s) - phalloidin , actin , thermal stability , förster resonance energy transfer , chemistry , biophysics , fluorescence , biology , biochemistry , cytoskeleton , physics , organic chemistry , optics , cell
In this work the effect of phalloidin and jasplakinolide on the dynamic properties and thermal stability of actin filaments was studied. Temperature dependent fluorescence resonance energy transfer measurements showed that filaments of Ca‐actin became more rigid in the presence of phalloidin or jasplakinolide. Differential scanning calorimetric data implied that the stiffer filaments also had greater thermal stability in the presence of phalloidin or jasplakinolide. The fluorescence and calorimetric measurements provided evidences that the extent of stabilization by jasplakinolide was greater than that by phalloidin.