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Regulation of the yeast phospholipid hydroperoxide glutathione peroxidase GPX2 by oxidative stress is mediated by Yap1 and Skn7
Author(s) -
Tsuzi Daisuke,
Maeta Kazuhiro,
Takatsume Yoshifumi,
Izawa Shingo,
Inoue Yoshiharu
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.03.091
Subject(s) - biochemistry , chemistry , oxidative stress , glutathione , glutathione peroxidase , microbiology and biotechnology , biology , superoxide dismutase , enzyme
The GPX2 gene encodes a homologue of phospholipid hydroperoxide glutathione peroxidase in Saccharomyces cerevisiae . The GPX2 promoter contains three elements the sequence of which is completely consistent with the optimal sequence for the Yap1 response element (YRE). Here, we identify the intrinsic YRE that functions in the oxidative stress response of GPX2 . In addition, we discovered a cis ‐acting element (5 ′ ‐GGCCGGC‐3 ′ ) within the GPX2 promoter proximal to the functional YRE that is necessary for H 2 O 2 ‐induced expression of GPX2 . We present evidence showing that Skn7 is necessary for the oxidative stress response of GPX2 and is able to bind to this sequence. We determine the optimal sequence for Skn7 to regulate GPX2 under conditions of oxidative stress to be 5 ′ ‐GGC(C/T)GGC‐3 ′ , and we designate this sequence the oxidative stress‐responsive Skn7 response element.