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Mapping of the insulin‐like growth factor II binding site of the Type I insulin‐like growth factor receptor by alanine scanning mutagenesis
Author(s) -
Sørensen Heidi,
Whittaker Linda,
Hinrichsen Jane,
Groth Andreas,
Whittaker Jonathan
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.03.077
Subject(s) - alanine scanning , alanine , biochemistry , chemistry , binding site , insulin like growth factor , receptor , site directed mutagenesis , insulin receptor substrate , insulin like growth factor 1 receptor , insulin receptor , mutagenesis , biology , growth factor , amino acid , insulin , endocrinology , mutation , insulin resistance , mutant , gene
The Type I insulin‐like growth factor receptor is a physiological receptor for insulin‐like growth factor II (IGF‐II). To characterize the molecular basis of the receptor's ligand binding properties, we have examined the effects of alanine mutations of residues in the ligand binding site of the receptor on its affinity for IGF‐II. The functional epitope for IGF‐II comprises residues in the N‐terminal L1 domain and residues at the C‐terminus of the α subunit. Cysteine rich domain residues do not appear to be critical for IGF‐II binding.