Open AccessScreening of diseases associated with abnormal metabolites for evaluation of HPLC in organic aciduria profiling
Author(s) -
Dina A. Ghoraba,
Mervat S. Mohamed,
Osama K. Zaki
Publication year - 2014
Publication title -
the egyptian journal of medical human genetics /the egyptian journal of medical human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.253
H-Index - 17
eISSN - 2090-2441
pISSN - 1110-8630
DOI - 10.1016/j.ejmhg.2013.11.005
Subject(s) - high performance liquid chromatography , chromatography , metabolite , urine , chemistry , newborn screening , mass spectrometry , tandem mass spectrometry , biochemistry
BackgroundOrganic acid disorders are a heterogeneous group of inborn errors of metabolism, in which organic acids accumulate in the body. They have high prevalence in Egypt because of a high rate of consanguineous marriages. Here we report our experience with the diagnostic evaluation of patients with organic acidemias as well as several other inborn errors of metabolism (IEMs) by liquid chromatography–tandem mass spectrometry (LC/MS–MS), gas-chromatography mass spectrometry (GC/MS) and by isocratic cation exchange “high-performance liquid-chromatography” (HPLC) to evaluate the use of HPLC method for disease-associated metabolite screening.Patients and methodsIn this study, we screened 86 suspected Egyptians patients with organic acid disorders by LC/MS–MS, GC/MS and by HPLC aged from 3days to 12years old. Data obtained from the three methods were statistically analyzed to evaluate the specificity and sensitivity of the HPLC method over the other two methods and to pursue its precision in the diagnosis of organic acid disorders. Moreover, 17 urine samples were collected from patients with several other IEMs to evaluate the efficiency of HPLC in detecting abnormal metabolites in urine samples.ResultsThe screening results showed that diagnostic efficiencies were varied among the three methods, HPLC showing a higher sensitivity of detecting normal urine as well as a highly satisfactory extent for the detection of different metabolic disorders. In addition, some typical urinary HPLC chromatograms of different metabolic disorders were presented to help the investigator who is going to start an organic aciduria screening program by HPLC to be familiar with various patterns.ConclusionThis study has indicated that HPLC is an easy applicable and useful technique for the initial screening of organic acid disorders and many other disease associated metabolites