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Combined therapy with human cord blood cell transplantation and basic fibroblast growth factor delivery for treatment of myocardial infarction
Author(s) -
Cho SeungWoo,
Kim IlKwon,
Bhang Suk Ho,
Joung Boyoung,
Kim Young Jin,
Yoo Kyung Jong,
Yang YoonSun,
Choi Cha Yong,
Kim ByungSoo
Publication year - 2007
Publication title -
european journal of heart failure
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.149
H-Index - 133
eISSN - 1879-0844
pISSN - 1388-9842
DOI - 10.1016/j.ejheart.2007.06.012
Subject(s) - medicine , basic fibroblast growth factor , neovascularization , transplantation , myocardial infarction , infarction , ejection fraction , cell therapy , angiogenesis , vascular endothelial growth factor , cord blood , cardiology , growth factor , surgery , heart failure , cell , vegf receptors , genetics , receptor , biology
Background: Transplanting cord blood‐derived cells has been shown to augment neovascularization in ischaemic tissue. Aim: To test whether sustained delivery of basic fibroblast growth factor (bFGF) enhances the efficacy of angiogenic cord blood mononuclear cell (CBMNC) transplantation therapy in treating myocardial infarction. Methods: Three weeks after myocardial infarction, Sprague‐Dawley rats were randomised to either injection of medium only (control), CBMNC transplantation, sustained bFGF delivery, or combined CBMNC transplantation and sustained bFGF delivery. Six weeks after treatment, tissue formation, neovascularization, and apoptotic activity in the infarct regions were evaluated by histology and immunohistochemistry. Left ventricular (LV) dimensions and function were evaluated by magnetic resonance imaging. Results: Combined bFGF delivery and CBMNC transplantation significantly enhanced neovascularization in the ischaemic myocardium, as compared with either therapy alone. The enhanced neovascularization was likely due to increased VEGF and bFGF expression. The combined therapy also exhibited a reduced infarct area and apoptosis in the ischaemic myocardium, as compared with either individual therapy. The combined therapy did not attenuate LV dilation or increase ejection fraction significantly over either individual therapy. Conclusion: This study demonstrates that sustained bFGF delivery enhances the angiogenic efficacy of CBMNC transplantation in rat myocardial infarction models.