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Granulocyte Macrophage‐Colony Stimulating Factor receptor expression on human cardiomyocytes from end‐stage heart failure patients
Author(s) -
Postiglione Loredana,
Montagnani Stefania,
Ladogana Paolo,
Castaldo Clotilde,
Di Spigna Gaetano,
Bruno Eugenia Maria,
Turano Mimmo,
De Santo Luca,
Cudemo Giuseppe,
Cocozza Sergio,
Divitiis Oreste,
Rossi Guido
Publication year - 2006
Publication title -
european journal of heart failure
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.149
H-Index - 133
eISSN - 1879-0844
pISSN - 1388-9842
DOI - 10.1016/j.ejheart.2005.12.007
Subject(s) - heart failure , medicine , myocyte , fibrosis , myocardial infarction , regeneration (biology) , cardiomyopathy , pathology , microbiology and biotechnology , cardiology , biology
Background In remodelling ventricles, the progression of heart failure is associated with structural changes involving the extra‐cellular matrix (ECM) and the cytoskeleton of cardiomyocytes, associated with fibrosis, cellular damage and death. The role of some cytokines and haematopoietic growth factors in the mechanism of both damage and regeneration of cardiac tissue during acute myocardial infarction has been demonstrated. Following heart damage, the development of scarred tissue was considered to be the only outcome, since myocytes were considered to be terminally differentiated cells. However, recent studies in animal models and adult human hearts have shown that myocytes can proliferate under the modulation of several factors. Aims To assess Granulocyte Macrophage‐Colony Stimulating Factor (GM‐CSF) receptor expression in healthy and diseased human hearts, and to evaluate the possible role of GM‐CSF and its receptor in the regeneration of cardiac tissue in chronic cardiomyopathy. Methods and results GM‐CSFR expression in human cardiac tissue from explanted hearts of ten patients with end‐stage heart failure and in cardiac biopsies from eight normal human hearts was studied by immunohistochemistry, and cellular and molecular biology assays. Our results demonstrated an increase in GM‐CSFR in cardiomyocytes from end‐stage heart failure tissues as compared to normal control tissues. Conclusions We hypothesize that GM‐CSF plays a role in apoptotic and/or ECM deposition processes as well as in cytoskeleton modification in the myocardium.