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Cardiac PPARα expression in patients with dilated cardiomyopathy
Author(s) -
Schupp Michael,
Kintscher Ulrich,
Fielitz Jens,
Thomas Jennifer,
Pregla Reinhard,
Hetzer Roland,
Unger Thomas,
RegitzZagrosek Vera
Publication year - 2006
Publication title -
european journal of heart failure
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.149
H-Index - 133
eISSN - 1879-0844
pISSN - 1388-9842
DOI - 10.1016/j.ejheart.2005.09.003
Subject(s) - medicine , dilated cardiomyopathy , carnitine , endocrinology , heart failure , peroxisome proliferator activated receptor , cardiomyopathy , beta oxidation , peroxisome , diabetic cardiomyopathy , gene expression , cardiology , receptor , metabolism , biology , gene , biochemistry
Background: The peroxisome proliferator‐activated receptor alpha (PPARα) is a central regulator of myocardial fatty acid (FA) metabolism implicated in the pathogenesis of heart failure. Aims: To characterize PPARα regulation in human dilated cardiomyopathy (DCM), we studied the expression of cardiac PPARα, cardiac carnitine palmitoyl‐transferase I (CPT‐1), a major PPARα target gene, and of the cardiac glucose transporter GLUT‐4 in patients with DCM. Methods: Left ventricular biopsies were taken from patients with DCM ( n =16) and control subjects ( n =15), and mRNA expression was quantitated using real‐time PCR (SYBR ® Green) and protein expression was measured by Western immunoblotting. Results: Left ventricular PPARα mRNA levels were significantly increased in the DCM group compared to the control group (136±25.4% vs. control, p <0.01). Consistently, DCM patients had a significantly higher cardiac CPT‐1 mRNA expression (147±51% vs. control, p <0.05) compared to the control group. Cardiac GLUT‐4 expression was similar in both groups. Conclusion: Elevated cardiac PPARα levels followed by an induction of cardiac CPT‐1 expression may result in increased fatty acid metabolism for cardiac energy production in DCM, suggesting a specific cardiac metabolic program in human DCM compared to other types of cardiomyopathy.