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Functional assessment of myoblast transplantation for cardiac repair with magnetic resonance imaging
Author(s) -
Bos Ewout J.,
Thompson Richard B.,
Wagner Anja,
Mahrholdt Heiko,
Morimoto Yoshihisa,
Thomson Louise E.J.,
Wang Lynn H.,
Duncker Dirk J.,
Judd Robert M.,
Taylor Doris A.
Publication year - 2005
Publication title -
european journal of heart failure
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.149
H-Index - 133
eISSN - 1879-0844
pISSN - 1388-9842
DOI - 10.1016/j.ejheart.2003.12.022
Subject(s) - medicine , cardiology , transplantation , myocardial infarction , magnetic resonance imaging , ejection fraction , myocyte , ventricular remodeling , intracardiac injection , end diastolic volume , infarction , thickening , heart failure , stroke volume , radiology , chemistry , polymer science
Background: Contraction of transplanted myoblasts and their effects on function and remodeling after myocardial infarction remain controversial. Aim: We used magnetic resonance imaging (MRI) to study wall thickening and left ventricular (LV) function and geometry after myoblast transplantation. Methods and results: Three weeks after cryo‐infarction rabbits were randomized to receive an injection of ∼2×10 8 myoblasts ( n =8) or medium ( n =9) into the scar. Cine MRI and contrast enhanced (ce) MRI images were acquired before injection (baseline) and 4 weeks later (endpoint). Regional wall thickening was measured at the site of transmural hyperenhancement. In the control group, regional wall thickening decreased to −15.3±8.6% at baseline, which further decreased to −18.3±5.7% at endpoint. Further, end‐diastolic volume increased from 3.96±0.27 to 5.00±0.46 ml and end‐systolic volume from 2.23±0.19 to 2.96±0.30 ml (both P <0.05 vs. baseline), which was accompanied by increased LV wall volumes ( P <0.05 vs. baseline). In contrast, myoblast transplantation increased regional wall thickening from −11.9±15.9% at baseline to 26.9±17.0% ( P <0.05 vs. control), which resulted in significantly improved two‐dimensional ejection fractions at the infarct level and prevented the increase in end‐diastolic and end‐systolic volumes and wall volume. Conclusion: Intracardiac myoblast transplantation after myocardial infarction improves regional wall thickening and prevents progressive left ventricular remodeling.