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Cardiac chimerism in recipients of peripheral‐blood and bone marrow stem cells
Author(s) -
BayesGenis Antoni,
MuñizDiaz Eduardo,
Catasus Lluis,
Arilla Marina,
Rodriguez Carmen,
Sierra Jordi,
Madoz Pedro J.,
Cinca Juan
Publication year - 2004
Publication title -
european journal of heart failure
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.149
H-Index - 133
eISSN - 1879-0844
pISSN - 1388-9842
DOI - 10.1016/j.ejheart.2003.12.006
Subject(s) - medicine , bone marrow , peripheral blood , stem cell , peripheral , progenitor cell , immunology , transplantation , somatic cell , heart failure , pathology , biology , genetics , gene
Multipotent progenitor cells have the ability to differentiate into most somatic cell types, including cardiac myocytes. We sought to investigate cardiac chimerism after peripheral‐blood and bone marrow stem cell transplantation. Between 10 and 17 highly polymorphic short tandem repeat (STR) markers were assayed in DNA obtained from donors’ peripheral blood, recipients’ peripheral blood before transplantation, and the recipient's heart in every patient. Gender and non‐gender STR donor alleles were identified in the recipient heart in three patients. Using a highly sensitive PCR assay to determine donor and recipient genotypes, we confirmed the existence of cardiac chimerism in recipients of peripheral‐blood and bone marrow stem cells.