Open AccessLaboratory cross-contamination of Mycobacterium tuberculosis in Northwest of Iran
Author(s) -
Mohammad Asgharzadeh,
Hossein Samadi Kafil,
Sajjad Ghorghanlu,
Mahya Pourostadi
Publication year - 2015
Publication title -
egyptian journal of chest diseases and tuberculosis/egyptian journal of chest diseases and tuberculosis
Language(s) - English
Resource type - Journals
eISSN - 2090-9950
pISSN - 0422-7638
DOI - 10.1016/j.ejcdt.2015.03.018
Subject(s) - genotyping , tuberculosis , mycobacterium tuberculosis , medicine , microbiology and biotechnology , gold standard (test) , veterinary medicine , contamination , restriction fragment length polymorphism , virology , polymerase chain reaction , biology , genotype , pathology , genetics , ecology , gene
Gold standard method for Mycobacterium tuberculosis identification is microbial culture, but this method can cause cross-contaminations. In the present study we aimed to investigate possible cross-contaminations in two main tuberculosis laboratories in Northwest of Iran.MethodsOne hundred and fifty-six isolates from two central tuberculosis laboratories were investigated by IS6110-RFLP and VNTR-ETR methods.Results53 isolates were clustered in 18 clusters. 15 isolates were smear negative and single culture positive among which four isolates were suspected to be contaminated and in two isolates (1.28%) contaminations were confirmed.ConclusionWith this confirmation clustered isolates reduced from 53 isolates to 49 isolates and recent transmission rate of tuberculosis reduced to 21.2%. This study showed genotyping smear negative and single culture positive isolates can prevent unnecessary treatments and for this purpose VNTR-ETR is a suitable method