Open AccessRelationship of apolipoprotein E polymorphism with lipid profiles in atherosclerotic coronary artery disease
Author(s) -
Ibrahim Elmadbouh,
Yasser Elghobashy,
Eman Abd–Allah,
Ashraf Reda,
Adnan Fathe,
Safaa I. Tayel,
Tarek Abd-Elhakim
Publication year - 2013
Publication title -
the egyptian heart journal /the egyptian heart journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.212
H-Index - 9
eISSN - 2090-911X
pISSN - 1110-2608
DOI - 10.1016/j.ehj.2012.11.002
Subject(s) - medicine , apolipoprotein e , coronary artery disease , allele , genotype , cardiology , genotyping , odds ratio , lipid profile , stenosis , apolipoprotein b , carotid artery disease , cholesterol , gastroenterology , disease , carotid endarterectomy , biology , gene , genetics
AimsThe aim was to determine the relationship between apolipoprotein E (ApoE) gene polymorphisms and lipid profile in patients with coronary artery diseases (CAD), and its role in the prediction of the severity of carotid and coronary atherosclerosis.Methods and resultsOne hundred patients were classified by coronary angiography: 80 patients with CAD and 20 controls (normal coronary angiography). Clinical data, carotid sonography, blood lipid profiles and ApoE genotyping (PCR-RFLP) were assessed. CAD patients had significantly increased plasma lipid profiles and carotid intimal-wall thickness (IMT) versus controls. In CAD patients; ApoE genotype frequencies were E3/E3=62.50%, E2/E3=18.75%, E3/E4=17.50%, E2/E4=1.25%, E4/E4=0 and E2/E2=0. But, E3/E4 genotype was significantly higher than controls (P<0.05). Also, in CAD patients; ApoE allele frequencies were E3=80.6%, E2=10.0% and E4=9.4% but, ApoE4 alleles were associated with higher cholesterol (P=0.034) and LDL-c (P=0.003), while ApoE2 alleles were associated with higher triglycerides (P=0.037) versus ApoE3 alleles. However, odds ratio of CAD patients had higher risk with E2/E3 genotypes (2.5-fold), E2 alleles (2.2-fold) and E4 alleles (2.1-fold). Moreover, CAD patients with ApoE4 alleles had significantly higher carotid IMT (1.23±0.26mm vs 0.97±0.2mm ApoE3, P=0.006; however, non-significant vs 1.10±0.40mm ApoE2 and also, ApoE2 vs ApoE3 alleles, P=0.633) and left anterior descending (LAD) coronary artery stenosis (vs ApoE3 alleles, P=0.016).ConclusionIschemic patients with carotid and coronary atherosclerosis had significantly higher integration of dyslipidemia and ApoE alleles (ApoE2 with hypertriglyceridemia and ApoE4 with hypercholesterolemia and higher LDL-c). ApoE polymorphism may be an important diagnostic risk biomarker and may implicate therapeutic intervention in atherosclerotic ischemic patients