Relative profile analysis of molecular markers for identification and genetic discrimination of loaches (Pisces, Nemacheilidae) | Zendy

Tejas S. Patil | Zendy; Asif S. Tamboli | Zendy; Swapnil M. Patil | Zendy; Amrut R. Bhosale | Zendy; Sanjay P. Govindwar | Zendy; Dipak V. Muley | Zendy

Open Access

Relative profile analysis of molecular markers for identification and genetic discrimination of loaches (Pisces, Nemacheilidae)

Author(s) -

Tejas S. Patil,

Asif S. Tamboli,

Swapnil M. Patil,

Amrut R. Bhosale,

Sanjay P. Govindwar,

Dipak V. Muley

Publication year - 2016

Publication title -

comptes rendus biologies

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.529

H-Index - 84

eISSN - 1768-3238

pISSN - 1631-0691

DOI - 10.1016/j.crvi.2016.06.001

Subject(s) - amplified fragment length polymorphism , biology , rapd , genetics , genetic diversity , molecular marker , phylogenetic tree , genetic marker , loss of heterozygosity , allele , gene , population , demography , sociology

Genus Nemacheilus, Nemachilichthys and Schistura belong to the family Nemacheilidae of the order Cypriniformes. The present investigation was undertaken to observe genetic diversity, phylogenetic relationship and to develop a molecular-based tool for taxonomic identification. For this purpose, four different types of molecular markers were utilized in which 29 random amplified polymorphic DNA (RAPD), 25 inter-simple sequence repeat (ISSR) markers, and 10 amplified fragment length polymorphism (AFLP) marker sets were screened and mitochondrial COI gene was sequenced. This study added COI barcodes for the identification of Nemacheilus anguilla, Nemachilichthys rueppelli and Schistura denisoni. RAPD showed higher polymorphism (100%) than the ISSR (93.75-100%) and AFLP (93.86-98.96%). The polymorphic information content (PIC), heterozygosity, multiplex ratio, and gene diversity was observed highest for AFLP primers, whereas the major allele frequency was observed higher for RAPD (0.5556) and lowest for AFLP (0.1667). The COI region of all individuals was successfully amplified and sequenced, which gave a 100% species resolution.

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