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PIII‐29
Author(s) -
ZakrzewskiJakubiak M.,
Denus S.,
Dubeé M.,
Beélanger F.,
White M.,
Turgeon J.
Publication year - 2006
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1016/j.clpt.2005.12.237
Subject(s) - genotype , medicine , bradykinin , population , enos , aldosterone , heart failure , allele frequency , angiotensin converting enzyme , gene polymorphism , gastroenterology , endocrinology , genotype frequency , biology , genetics , receptor , gene , nitric oxide synthase , environmental health , nitric oxide , blood pressure
BACKGROUND/AIMS Certain studies suggest a difference in genetic polymorphism frequencies between healthy and heart failure (HF) patients. The aim of this study was to verify if such a difference exists in ten polymorphisms of RAAS in our population. METHODS This is a case‐control study performed on 200 healthy volunteers and 58 HF patients. The healthy control group consisted of males aged between 18 and 25 years. The HF group were between 50 and 65 years old, in NYHA class II‐IV HF and were recruited in a tertiary care hospital. Both groups were from French‐Canadian origin. The analysed polymorphisms were: ACE I/D, ATR1 A1166C, AGT M235T, AGT T174M, eNOS T‐786C, eNOS G298A, Beta‐2 adrenergic receptor Q27E, Bradykinin β2R +9/−9, CYP11B2 T‐344C and α‐adducin G460W. The Gen Elute Blood Genomic DNA kit (Sigma PC# NA2020) was used to extract DNA. The polymorphisms were then analysed by the standard PCR/enzymatic digestion/electrophoresis method. RESULTS All the polymorphisms tested were in Hardy‐Weinberg equilibrium, except for ATR1 in our HF group. We obtained a statistically significant difference in the genotype frequency of the AGT235 gene, where the study group contained more T/T (mutant allele) and less M/M homozygotes. The difference in genotype frequency reached borderline significance with respect to the AGT174 and the bradykinin β2R polymorphisms. CONCLUSION This study demonstrates than the AGT235 polymorphism may be associated with HF in a French‐Canadian population. Clinical Pharmacology & Therapeutics (2005) 79 , P66–P66; doi: 10.1016/j.clpt.2005.12.237