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Nanocrystalline silver inhibits antibiotic‐, antiseptic‐resistant bacteria
Author(s) -
Lyczak J. B.,
Schechter P. J.
Publication year - 2005
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1016/j.clpt.2004.12.119
Subject(s) - antiseptic , microbiology and biotechnology , bacteria , pseudomonas aeruginosa , minimum inhibitory concentration , antibiotics , chemistry , antibacterial activity , benzalkonium chloride , tetracycline , antibiotic resistance , bacterial growth , biology , chromatography , genetics , organic chemistry
Background/Aims Resistance to antibacterial agents is increasingly prevalent. This study evaluated the activity of nanocrystalline silver (NCS, crystallite < 50nm), produced by physical vapor deposition, against bacteria resistant to either antibiotics or the antiseptic benzalkonium chloride (BAC). Methods Minimal Inhibitory Concentration (MIC) assay; NCS was serially diluted; bacteria were added and incubated overnight at 37 °C; growth of bacteria was assessed by optical density of the cultures. Results The MIC of NCS against multi‐resistant isolates of Burkholderia dolosa was 6±0.8 (s. e.) ug/ml, and of B. multivorans was 6±1.0 ug/ml. This compared to an MIC of 7±0.9 ug/ml for other Gram‐negatives tested. Additionally, the activity of NCS (MIC=3 ug/ml) against Pseudomonas aeruginosa (PA) with artificially‐introduced tetracycline resistance was comparable to its activity (MIC=4 ug/ml) against the non‐resistant parent strain. Lastly, NCS was effective (MIC=6 ug/ml) against a clinical isolate of PA resistant to BAC. Conclusions NCS effectively inhibits the growth of bacteria with natural resistance and artificially‐introduced resistance to antibiotics, and is also effective against antiseptic‐resistant PA. NCS is a promising candidate for development of future antibacterial therapies. Clinical Pharmacology & Therapeutics (2005) 77 , P60–P60; doi: 10.1016/j.clpt.2004.12.119