Premium
Association between CYP3A5 and blood pressure in nigerian males
Author(s) -
Neville K. A.,
Ayaji O. E.,
Holland C. L.,
Skaar T. C.,
Hall S. D.
Publication year - 2005
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1016/j.clpt.2004.12.099
Subject(s) - blood pressure , genotype , cyp3a5 , allele , medicine , endocrinology , biology , gastroenterology , genetics , gene
Background Recent data suggest that the Cytochrome P4503A5 (CYP3A5) genotype may play a role in hypertension in African‐Americans (Givens R, et al., JAppl Physiol 2003; 95:1297–300). Data have also shown that CYP3A5 is expressed in the kidney and may play a role in renal sodium retention (Haehner BD, et al., Mol Pharmacol 1996; 50:52–59). Methods We determined the CYP3A5 genotype and measured blood pressure by manual sphygmomanometry in 105 Nigerian subjects. Genomic DNA was extracted from buccal washings. Each subject's age, height, weight, pulse rate, and blood pressure was recorded. Real Time‐PCR was performed on the samples to determine the CYP3A5 genotype. Results 68% of the subjects were homozygous for the wild‐type allele (*1/*1), 31% were heterozygous (*1/*3), and 1% were homozygous for the reduced‐activity allele (*3/*3). The frequency distributions of the data are in Hardy‐Weinberg equilibrium (see Table). The study had greater than 80% power to detect a 5 mmHg or greater difference between groups with respect to blood pressure and no significant difference was detected. The *3/*3 group was not used for comparison since it contained only 1 subject. Conclusions A correlation between CYP3A5 genotype and blood pressure has previously been suggested in literature. However, in this study no significant difference was detected between homozygous (*1/*1) and heterozygous (*1/*3) groups for blood pressure or any other clinical parameter tested. Clinical Pharmacology & Therapeutics (2005) 77 , P55–P55; doi: 10.1016/j.clpt.2004.12.099 Average Clinical Results based on CYP3A5 genotype of 105 Nigerian males. The study had greater than 80 power to detect a 5 mmHg difference in blood pressureCYP3A5 Genotype Number of subjects (n = 106) Age Height (m ± SD) Weight (kg ± SD) Body Mass Index (BMI ± SD) Systolic Blood Pressure (mmHg ± SD) Diastalic Blood Pressure (mmHg ± SD)*1 *1 71 23.3 ± 2.7 1.72 ± 0.1 64.2 ± 10.4 21.6 ± 2.8 112 ± 15 72 ± 10 *1 *3 33 24.4 ± 2.6 1.71 ± 0.1 65.9 ± 13.1 22.3 ± 3.3 113 ± 11 72 ± 11 *3 *3 1 N/A N/A N/A N/A N/A N/A p‐value for comparison of values between *1 *1 and *1 *3 groups 0.14 0.60 0.33 0.18 0.73 0.79