Premium
Effect of semen sampling frequency on concentrations of zidovudine and male genital tract glandular markers, fructose, PSA, and sperm count
Author(s) -
Ndovi T. T.,
Lee J. I.,
Hamzeh F.,
Hendrix C. W.
Publication year - 2004
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1016/j.clpt.2003.11.055
Subject(s) - semen , sperm , blood sampling , fructose , sampling (signal processing) , confidence interval , zidovudine , medicine , andrology , physiology , biology , immunology , human immunodeficiency virus (hiv) , biochemistry , viral disease , filter (signal processing) , computer science , computer vision
The study of antiretroviral drugs in semen is of interest in understanding the relationship between drug concentration and HIV transmission/resistance risk. The ability to sample semen more frequently than the traditional 48–72 hr interval would greatly facilitate these PK studies. We studied the effect of sampling interval on semen drug concentration. Six healthy subjects received continuous IV infusions of zidovudine (ZDV) to maintain steady state and exclude blood concentration as an experimental variable. ZDV concentration was measured in paired semen and blood samples collected at diminishing time intervals (48, 24, 12, 8, 4, 2, 1 hrs) over a 5 day period. We also measured seminal volume, PSA, fructose, and sperm counts in semen. All parameters were tested for changes related to sampling interval. All but 1 subject submitted all 7 paired semen and blood samples. Zidovudine S:B ratios were not significantly different (p = 0.60) across sampling time intervals, but were highly variable, ranging from 1.33 to 20.7 (median 3.4). Semen volume and fructose concentration significantly decreased (p=0.003 and 0.001, respectively) at sampling intervals < 4 hrs. PSA and sperm counts did not change with sampling interval. We have shown that 1 hour sampling intervals are feasible. ZDV S:B ratios, while not affected by 1 hour sampling intervals, were so variable as to limit our ability to detect small changes. Fructose, a seminal vesicle marker, is decreased by sampling this frequently. Clinical Pharmacology & Therapeutics (2004) 75 , P15–P15; doi: 10.1016/j.clpt.2003.11.055