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Silencing of calpain expression reduces the metastatic potential of human osteosarcoma cells
Author(s) -
Fan DeGang,
Dai JingYao,
Tang Juan,
Wu MingMei,
Sun SiGuo,
Jiang JianLi,
Fan QingYu
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2009.08.014
Subject(s) - osteosarcoma , calpain , gene silencing , metastasis , cancer research , transfection , proteases , matrix metalloproteinase , cell culture , biology , medicine , chemistry , pathology , cancer , enzyme , gene , biochemistry , genetics
Osteosarcoma, the most common primary bone tumor in young adults, is characterized by local invasion and distant metastasis. But detailed mechanisms of tumorigenicity and metastasis of osteosarcoma are not well known. We report the involvement of calpains, a family of calcium‐activated, cysteine proteases, in the invasive and metastatic processes of human osteosarcoma cells. By using siRNA treatment, the expression of μ‐ and m‐calpains were downregulated in human Saos‐2 osteosarcoma cells. Both the adhesive and invasive potentials were significantly attenuated in calpain siRNA‐transfected human Saos‐2 osteosarcoma cells. MMPs are the main factors involved in malignant tumor invasion and metastasis. siRNA of calpains also significantly inhibited the secretion of MMP‐2 in Saos‐2 cells. These results suggest that μ‐ and m‐calpains are important in the invasion and metastasis of human osteosarcoma cells, and calpains might be targeted to reduce tumor progression.