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Generation and characterization of functional cardiomyocytes using induced pluripotent stem cells derived from human fibroblasts
Author(s) -
Gai Hui,
Leung Elaine LaiHan,
Costantino Peter D.,
Aguila Jerell R.,
Nguyen David M.,
Fink Louis M.,
Ward David C.,
Ma Yupo
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2009.08.008
Subject(s) - induced pluripotent stem cell , embryonic stem cell , foreskin , microbiology and biotechnology , cellular differentiation , biology , immunostaining , stem cell , in vitro , cell culture , immunology , biochemistry , immunohistochemistry , genetics , gene
Abstract We have successfully developed both spontaneous and inductive cardiomyocyte differentiation of iPS cells reprogrammed from human foreskin fibroblasts. The reprogrammed iPS cells morphologically resemble human cardiomyocytes which can beat. RT‐PCR and immunostaining show that cardiac markers are expressed that are comparable to the differentiation pattern of authentic human embryonic stem cells, indicating the existence of both immature and mature differentiated cardiomyocytes. 5‐Azacytidine greatly enhanced the efficiency of cardiomyocyte differentiation, whereas dimethylsulfoxide had no effect. Low serum and bone morphogenetic protein‐2 marginally improved differentiation efficiency. iPS cell‐derived cardiomyocytes changed their beat frequency in response to cardiac drugs, which included ion channel blockers and α/β adrenergic stimulators. Derived cardiomyocytes look promising as an in vitro system for potential drug screen and/or toxicity, making this system closer to practical use in the near future.