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Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow
Author(s) -
Cho KyungAh,
Ju SunYoung,
Cho Su Jin,
Jung YunJae,
Woo SoYoun,
Seoh JuYoung,
Han HoSeong,
Ryu KyungHa
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2009.04.023
Subject(s) - mesenchymal stem cell , regeneration (biology) , stem cell , bone marrow , haematopoiesis , flow cytometry , stem cell transplantation for articular cartilage repair , biology , microbiology and biotechnology , pathology , liver regeneration , liver injury , adult stem cell , cellular differentiation , immunology , medicine , endocrinology , biochemistry , gene
We have previously reported that bone marrow cells (BMCs) participate in the regeneration after liver injury. However, it is not established that this is the result of differentiation of hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) or the combination of both. We investigated the contribution of each cell fraction to the regenerative process. First, we confirmed that transplanted stem cells migrate directly to injured liver tissue without dispersing to other organs. Next, we divided green fluorescent protein (GFP)‐expressing BMCs into three populations as mononuclear cells, MSCs and HSCs. We then compared the engraftment capacity after transplantation of each fraction of cells into liver‐injured mice. Of these, the MSCs transplanted group showed the highest GFP fluorescence intensities in liver tissue by flow cytometry analysis and confocal microscopic observation. Furthermore, MSCs showed differentiation potential into hepatocytes when co‐cultured with injured liver cells, which suggests that MSCs showed highest potential for the regeneration of injured liver tissue compared with those of the other two cell refractions.