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Proteomics and transcriptome approaches to investigate the mechanism of human sex determination
Author(s) -
Sato Youichi,
Shinka Toshikatsu,
Chen Gang,
Yan HongTao,
Sakamoto Kozue,
Ewis Ashraf A.,
Aburatani Hiroyuki,
Nakahori Yutaka
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2009.04.019
Subject(s) - testis determining factor , transcriptome , biology , fgf9 , y chromosome , microbiology and biotechnology , sertoli cell , transcription factor , proteomics , gene , gene expression , genetics , spermatogenesis , endocrinology
The SRY gene (sex‐determining region on the Y chromosome) was isolated in 1990 and is known as the testis‐determining factor on the Y chromosome. The SRY has been considered as a transcription factor since it contains an HMG box, which functions as a DNA‐binding domain. However, a direct target for SRY remains to be identified. We have investigated the function of SRY through proteomics and transcriptome approaches, and by using two stable SRY‐overexpressing cell lines (SRY1 and SRY2) in NT2/D1 cells derived from human testicular embryonal cell carcinoma. The results of 2‐dimensional gel electrophoresis show that SRY overexpression causes a considerable downregulation of many chaperone proteins. SRY also upregulates laminin, which is important for Sertoli cell differentiation. Additionally, transcriptome analysis shows that SRY overexpression upregulates many zinc finger proteins and downregulates cellular growth factors with S or G 2 /M arrest of the cell cycle and inhibition of cellular proliferation.