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Effect of silicon dioxide on expression of poly (ADP‐Ribose) polymerase mRNA and protein
Author(s) -
Gao Ai,
Song Shanshan,
Wang Danlin,
Peng Wei,
Tian Lin
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2009.04.010
Subject(s) - poly adp ribose polymerase , hela , messenger rna , viability assay , silicon dioxide , microbiology and biotechnology , chemistry , real time polymerase chain reaction , parp inhibitor , polymerase , ribose , carcinogen , cell , biology , biochemistry , enzyme , materials science , gene , metallurgy
Silicon dioxide induces acute injury and chronic pulmonary fibrosis. International Agency for Research on Cancer (IARC) listed it as a human carcinogen in 1996. However, the molecular mechanisms to induce cancer are not understood yet. The content of poly (ADP‐ribose) polymerases (PARP) mRNA and protein in Hela cells treated with concentrations of silicon dioxide up to 400 μg/ml was determined by real‐time fluorogenetic quantitative PCR (RQ‐PCR) and immunofluorescence assay, respectively. MTT assay was used to determine cell viability. The results showed that viability at 400 μg/ml silica was significantly decreased but not at lower concentrations. The protein content of γ‐H2AX in silica‐treated group was significantly higher than the controls. The PARP mRNA and protein levels were significantly reduced with a dose response manner from the lowest silicon dioxide level. Our findings suggested that silicon dioxide increased the expression of γ‐H2AX and inhibited the expression of PARP mRNA and protein in Hela cells.