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DIXDC1 co‐localizes and interacts with γ‐tubulin in HEK293 cells
Author(s) -
Wu Yanrui,
Jing Xiaotang,
Ma Xin,
Wu Yan,
Ding Xuefeng,
Fan Wenhong,
Fan Ming
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2009.04.001
Subject(s) - hek 293 cells , centrosome , microbiology and biotechnology , tubulin , mitosis , immunoprecipitation , interphase , biology , xenopus , midbody , microtubule , chemistry , cell cycle , cell culture , cell , genetics , cell division , gene , cytokinesis
DIXDC1 is a Dishevelled‐Axin (DIX) domain‐containing protein involved in neural development and Wnt signaling pathway. Besides the DIX domain, DIXDC1 also contains a coiled‐coil domain (MTH domain), which is a common feature of centrosomal proteins. We have demonstrated that exogenously expressed GFP‐tag fused DIXDC1 co‐localize with γ‐tubulin both at interphase and mitotic phase in HEK293 cells. By immunostaining with anti‐DIXDC1 and anti‐γ‐tubulin antibody, endogenous DIXDC1 was also co‐localized with γ‐tubulin at the centrosomes in HEK293 cells. We confirmed this interaction of DIXDC1 with γ‐tubulin by co‐immunoprecipitation. The findings suggest that DIXDC1 might play an important role in chromosome segregation and cell cycle regulation.