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Induction of genomic instability in cultured human colon epithelial cells following exposure to isocyanates
Author(s) -
Mishra Pradyumna Kumar,
Bhargava Arpit,
Raghuram Gorantla Venkata,
Jatawa Suresh Kumar,
Akhtar Nabila,
Khan Saba,
Tiwari Archana,
Maudar Kewal Krishan
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2009.03.008
Subject(s) - genome instability , microsatellite instability , apoptosis , dna damage , microbiology and biotechnology , biology , cell culture , genomic dna , phosphorylation , dna , gene , chemistry , genetics , microsatellite , allele
The toxic response of cultured human colon epithelial‐FHC cells to methyl isocyanate was investigated with regard to genomic instability. Qualitative and quantitative assessments of the extent of phosphorylation of DNA damage signaling factors such as ATM, γH2AX and p53, was increased in treated cells compared to controls. At the same time, many treated cells were arrested at the G2/M phase of the cell cycle, and had an elevated apoptotic index and increased inflammatory cytokine levels. Cytogenetic analyses revealed varied chromosomal anomalies, with abnormal expression of pericentrin protein. Analysis through ISSR PCR demonstrated increased microsatellite instability. The results imply that isocyanates can cause genomic instability in colonocytes.