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In vivo non‐enzymatic repair of DNA oxidative damage by polyphenols
Author(s) -
Tan Xiaorong,
Zhao Chenyang,
Pan Jing,
Shi Yimin,
Liu Guoan,
Zhou Bo,
Zheng Rongliang
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2009.03.005
Subject(s) - rutin , quercetin , dna damage , biochemistry , enzyme , chemistry , dna repair , polyphenol , dna , oxidative damage , antioxidant , in vivo , oxidative phosphorylation , biology , genetics
The non‐enzymatic repair of DNA oxidative damage can occur in a purely chemical system, but data show that it might also occur in cells. Human hepatoma cells (SMMC‐7721) and human hepatocyte cells (LO2) were treated with 200 μM H 2 O 2 for 30 min to induce oxidative DNA damage quantified by amount of 8‐OHdG and degree of DNA strand breaks, without inducing enzymatic repair. The dynamics of enzymatic repair activity quantified by unscheduled DNA synthesis, within 30 min after removal of H 2 O 2 enzymatic repair mechanism has not been initiated. However, pre‐incubation with low micromolar level polyphenols, quercetin or rutin can significantly attenuate DNA damage in both cell lines, indicating that the polyphenols did not work through an enzymatic mechanism. Unscheduled DNA synthesis after removal of H 2 O 2 was also markedly decreased by quercetin and rutin. Combined with our previous studies of fast reaction chemistry, the inhibitory effect of polyphenols have to be assigned to non‐enzymatic repair mechanism rather than to enzymatic repair mechanism or antioxidant mechanism.