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Rat marrow‐derived mesenchymal stem cells developed in a medium supplemented with the autologous versus bovine serum
Author(s) -
Eslaminejad Mohamadreza Baghaban,
Rouhi Leila,
Arabnajafi Mahmood,
Baharvand Hossein
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2009.03.001
Subject(s) - fetal bovine serum , mesenchymal stem cell , doubling time , bone marrow , andrology , stem cell , chemistry , population , immunology , cell culture , biology , microbiology and biotechnology , cell , biochemistry , medicine , genetics , environmental health
The controversial effect of autologous serum (AS) on human mesenchymal stem cells (MSC) was studied in rat MSC culture. Rat bone marrow cells were plated in a medium containing either FBS (fetal bovine serum) or AS were cultured to passage 3, during which the population doubling number (PDN) of both cultures were measured and compared statistically. The number of viable cells, the cell colonogic activity, and cell growth rate were also compared. In addition, mineralization in the osteogenic cultures from each system was measured. Our data indicated that AS enriched medium provided a microenvironment in which growth rate as well as bone differentiation of the isolated MSCs were significantly higher than in FBS enriched medium.