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Chronological expression of Wnt target genes Ccnd1 , Myc , Cdkn1a , Tfrc , Plf1 and Ramp3
Author(s) -
Röhrs Sonja,
Kutzner Nadine,
Vlad Annica,
Grunwald Thomas,
Ziegler Slava,
Müller Oliver
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2009.01.016
Subject(s) - wnt signaling pathway , cyclin d1 , expression (computer science) , gene , biology , genetics , cell cycle , computer science , programming language
The canonical Wnt pathway regulates several biological processes including development, cell growth and proliferation via consecutive gene regulation. A high number of target genes of the Wnt pathway has been identified, but the chronological order of target gene expression is still elusive. This order is supposed to be crucial for the controlled course of events downstream of the activated Wnt pathway. Here we present the expression chronologies of the target genes Ccnd1 (encoding for cyclin D1), Myc (c‐Myc), Cdkn1a (p21 CIP1/WAF1 ), Tfrc (Transferrin receptor 1), Plf1 (Proliferin‐1) and Ramp3 (Receptor activity‐modifying protein 3) in C57MG cells after stimulation with Wnt‐3a. We discriminated between immediate (below 1 h), early (between 1 and 6 h), intermediate (between 6 and 12 h) and late (after 12 h) targets. According to this classification Myc and Tfrc belong to the immediate target genes; Ccnd1 , Plf1 and Ramp3 are early target genes and Cdkn1a is an intermediate target gene.