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Electrical impedance measurements predict cellular transformation
Author(s) -
Park Giljun,
Choi Chang K.,
English Anthony E.,
Sparer Tim E.
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2009.01.013
Subject(s) - cxc chemokine receptors , transformation (genetics) , receptor , microbiology and biotechnology , g protein coupled receptor , cell growth , chemokine receptor , chemistry , cancer cell , cellular model , signal transduction , biophysics , chemokine , biology , cancer , biochemistry , in vitro , genetics , gene
Cellular transformation is the first step in cancer development. Two features of cellular transformation are proliferation in reduced serum and loss of contact inhibition. Electronic Cell‐Substrate Impedance Sensing (ECIS) measurements have been used to measure cellular proliferation, cytotoxicity, apoptosis, and attachment. We have used impedance measurements to distinguish normal cells from cells transformed with a constitutively active chemokine receptor, CXCR2. CXCR2, a member of the G‐protein coupled receptor (GPCR) family, is normally involved in cellular activation and migration, but a single amino acid substitution leads to constitutive activity. NIH3T3 cells were transformed with a constitutively active CXCR2 (D143V_CXCR2) and growth in reduced serum and foci formation were measured using established biological assays and compared to data from ECIS. The results of this study show that impedance measurements provide a quick and reliable way of measuring cellular transformation and provide real time assessment of transformed cellular parameters. Use of the ECIS system could allow a rapid screening of anti‐cancer drugs that alter cellular transformation.