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Isolation and expansion of equine umbilical cord‐derived matrix cells (EUCMCs)
Author(s) -
Passeri Simona,
Nocchi Francesca,
Lamanna Roberta,
Lapi Simone,
Miragliotta Vincenzo,
Giannessi Elisabetta,
Abramo Francesca,
Stornelli Maria Rita,
Matarazzo Micheletino,
Plenteda Daniele,
Urciuoli Patrizia,
Scatena Fabrizio,
Coli Alessandra
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.10.012
Subject(s) - umbilical cord , doubling time , epidermal growth factor , andrology , population , matrix (chemical analysis) , stem cell , microbiology and biotechnology , chemistry , immunology , biology , medicine , cell culture , cell , biochemistry , chromatography , genetics , environmental health
Stem cells from extra‐embryonic sources can be obtained by non‐invasive procedures. We have standardized a method for the expansion of equine umbilical cord‐derived matrix cells (EUCMCs) for potential therapy. EUCMCs were isolated from the umbilical cord of five mares immediately after delivery. For expansion, cells were grown in α‐MEM and MSCBM. Moreover, to measure the effect of growth factor supplementation, epidermal growth factor (EGF) was added to α‐MEM. α‐MEM and MSCBM media performed similarly in terms of population doubling and CFU number value. EGF supplementation of α‐MEM determined a significant increase of the population doubling value. EGF supplementation did not affect the adipogenic and chondrogenic differentiation while bone nodule sizes an increased with the osteogenic protocol. Both α‐MEM and MSCBM can be used to cultivate EUCMCs. α‐MEM supplemented with EGF might represent an advantage for EUCMCs expansion. The results could be useful in choosing the culture medium since α‐MEM is more cost‐effective than MSCBM.