Premium
Anti‐cancer effect of Cassia auriculata leaf extract in vitro through cell cycle arrest and induction of apoptosis in human breast and larynx cancer cell lines
Author(s) -
Prasanna R.,
Harish C.C.,
Pichai R.,
Sakthisekaran D.,
Gunasekaran P.
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.10.006
Subject(s) - apoptosis , mcf 7 , cell cycle , cancer cell , biology , cell culture , cancer research , growth inhibition , cell growth , cancer , cell cycle checkpoint , programmed cell death , dna fragmentation , human breast , biochemistry , genetics
Abstract The in vitro anti‐cancer effect of Cassia auriculata leaf extract (CALE) was evaluated in human breast adenocarcinoma MCF‐7 and human larynx carcinoma Hep‐2 cell lines. CALE preferentially inhibited the growth of both the cell lines in a dose‐dependent manner with IC 50 values of 400 and 500 μg for MCF‐7 and Hep‐2 cells, respectively. The results showed the anti‐cancer action is due to nuclear fragmentation and condensation, associated with the appearance of A 0 peak in cell cycle analysis that is indicative of apoptosis. In addition, CALE treated MCF‐7 and Hep‐2 cells had decreased expression of anti‐apoptotic Bcl‐2 protein and increased expression of pro‐apoptotic Bax protein, eventually leading a decrease in the Bcl‐2/Bax ratio. These results demonstrated that CALE inhibits the proliferation of MCF‐7 and Hep‐2 cells through induction of apoptosis, making CALE a candidate as new anti‐cancer drug.