Premium
Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver
Author(s) -
Ju SunYoung,
Cho KyungAh,
Cho Su Jin,
Jung YunJae,
Woo SoYoun,
Seoh JuYoung,
Han HoSeong,
Ryu KyungHa
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.10.002
Subject(s) - cxcr4 , bone marrow , hypoxia (environmental) , apoptosis , chemokine , stem cell , haematopoiesis , microbiology and biotechnology , chemistry , immunology , cancer research , andrology , medicine , biology , oxygen , inflammation , biochemistry , organic chemistry
Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20% for 24 h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia‐treated BMCs and their migration in response to SDF‐1α was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl 4 ‐injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF‐1α, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl 4 damaged liver.