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Different culture conditions used for arresting the G0/G1 phase of the cell cycle in goldfish ( Carassius auratus ) caudal fin‐derived fibroblasts
Author(s) -
Choresca Casiano H.,
Koo Ok Jae,
Oh Hyun Ju,
Hong So Gun,
Gomez Dennis K.,
Kim Ji Hyung,
Lee Byeong Chun,
Park Se Chang
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.09.015
Subject(s) - carassius auratus , cell cycle , biology , cell synchronization , microbiology and biotechnology , embryo , andrology , fish fin , cell , starvation , cell culture , confluence , fibroblast , medicine , endocrinology , fish <actinopterygii> , biochemistry , fishery , genetics , computer science , programming language
One of the most important factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We investigated the effects of serum starvation, culturing to confluence and roscovitine treatment on the cell cycle synchronization of goldfish caudal fin‐derived fibroblasts by flow cytometric analysis. The results show that culturing the cells to confluence (85.5%) and roscovitine treatment (82.71%) yield a significantly higher percentage of cells arrested in the G0/G1 ( P < 0.05) phase than serum starvation (62.85%). Different concentrations of roscovitine (5, 10, or 15 μM) induce cell cycle arrest at the G0/G1 phase.