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Effect of 60 Hz electromagnetic fields on the activity of hsp70 promoter: An in vitro study
Author(s) -
Rodríguez de la Fuente Abraham O.,
AlcocerGonzález Juan M.,
HerediaRojas Antonio J.,
BalderasCandanosa Isaías,
RodríguezFlores Laura E.,
RodríguezPadilla Cristina,
TamézGuerra Reyes S.
Publication year - 2009
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.09.014
Subject(s) - luciferase , hela , hsp70 , plasmid , microbiology and biotechnology , transfection , reporter gene , gene expression , gene , in vitro , expression vector , chemistry , biology , recombinant dna , heat shock protein , biochemistry
We have evaluated the effect of 60 Hz sinusoidal magnetic fields (MF) at 8 and 8 μT on expression of the luciferase gene contained in a gene construct labelled as Electromagnetic Field‐plasmid (pEMF). The vector included the hsp70 promotor containing the 3 nCTCTn sequences previously described for the induction of hsp70 expression by magnetic fields, as well as the reporter of the luciferase gene. We also replicated the study of Lin et al. [Lin H, Blank M, Rossol‐Haseroth K, Goodman R. Regulating genes with electromagnetic response elements. J Cell Biochem 2001;81(1):143–48]. The pEMF plasmid was transfected into HeLa and BMK16 cell lines that were later exposed to either MF or thermal shock (TS). An increased luciferase expression was found in both the cells exposed to MF and TS compared with their control groups ( P < 0.05). Furthermore, the combined effect of MF and TS was also analyzed. A synergistic effect between two factors was observed for this co‐exposure condition in terms of luciferase gene expression.