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A potential model for studying the plasticity and reprogramming of human epidermal stem cells through preimplantation blastocyst microinjection
Author(s) -
Jiang Ruzhang,
Huang Bing,
Jin Chenjin,
Song Ge,
Zhong Xiufeng,
Yuan Jing,
Xiang Peng,
He Yuan,
Liu Bingqian,
Sun Xuerong,
Zhang Yuehong,
Ge Jian
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.09.004
Subject(s) - microinjection , reprogramming , microbiology and biotechnology , homeobox protein nanog , biology , blastocyst , stem cell , induced pluripotent stem cell , embryonic stem cell , embryo , embryogenesis , genetics , cell , gene
Microinjection of adult stem cells (ASCs) into blastocysts provides a classic model for studying ASC plasticity. To explore the molecular mechanisms that govern the reprogramming of ASCs, we evaluated the experimental model through microinjection of human epidermal stem cells (hEpiSCs) into mouse blastocysts. Mouse blastocysts underwent regular embryogenesis after microinjection of allogeneic cells, confirmed by morphological observation and embryo cell counting. hEpiSCs survive and integrate into mouse embryos, by monitoring the migration of injected cells at 2, 4, 12, 16 and 24 h. In this xenogeneic system, hEpiSCs could be reprogrammed within 24 h, as evidenced by the silencing of CK15 and Integrinβ1 gene expression, without activation of Oct4 and Nanog . Microinjection of hEpiSCs into mouse blastocysts provides an efficient model for studying the molecular mechanisms of their plasticity. Moreover, the possibility of inducing pluripotent stem cells without transgenes or viruses can be entertained.