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Stimulation of dorsal root ganglion neurons activity by pancreatic cancer cell lines
Author(s) -
Li JunHui,
Ma QingYong,
Shen SuGang,
Hu HengTong
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.08.022
Subject(s) - dorsal root ganglion , neurite , neurofilament , nissl body , stimulation , cell culture , biology , pancreatic cancer , medicine , endocrinology , staining , microbiology and biotechnology , anatomy , immunohistochemistry , dorsum , cancer , in vitro , immunology , biochemistry , genetics
Stimulation of mice dorsal root ganglion neurons (DRGNs) activity by human pancreatic cancer (PanCa) cell line Mia PaCa‐2 and its potential molecule mechanism has been investaged. DRGNs were cultured alone or along with the MIA PaCa‐2. The effects of MIA PaCa‐2 to DRGNs were determined by neurofilament (NF) immunocytochemical and Nissl staining. ELISA was used to detect the concentration of insulin‐like growth factor‐1 (IGF‐1) in the culture supernatant. Cyton size, neurite outgrowth and neuronal activity in the experimental group were greater than in the control groups. However, the concentration of IGF‐1 in the supernatants was not significantly different from those in the blank and non‐cultured medium groups. In the presence of MIA PaCa‐2 cell line, cyton size, neurite outgrowth and neuronal activity were enhanced, which may provide more routes for the invasion of cancer cells along nerves.