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Hepatic differentiation of cord blood‐derived multipotent progenitor cells (MPCs) in vitro
Author(s) -
Moon Young Joon,
Lee Myoung Woo,
Yoon Hee Hoon,
Yang Mal Sook,
Jang In Keun,
Lee Jong Eun,
Kim Hyo Eun,
Eom Youngwoo,
Park Joon Seong,
Kim Hugh C.,
Kim Young Jin,
Lee KwangHo
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.07.017
Subject(s) - progenitor cell , biology , umbilical cord , stem cell , haematopoiesis , microbiology and biotechnology , western blot , cytokeratin , cord blood , immunology , biochemistry , immunohistochemistry , gene
Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells that possesses practical and ethical advantages. We previously reported a novel UCB‐derived adult stem cells which we termed umbilical cord blood‐derived multipotent progenitor cells’ (MPCs). MPCs were capable of differentiating into functional neuronal cells. Under appropriate conditions lasting several days or weeks, we now show that the MPCs differentiate into hepatocyte‐like cells in vitro ; their properties were verified using reverse transcription‐polymerase chain reaction (RT‐PCR), Western blot, immunofluorescence, periodic acid‐Schiff (PAS) staining of accumulated glycogen and an enzyme‐linked immunosorbent assay (ELISA). We also found that hepatic differentiated cells expressed hepatocyte specific markers, such as albumin, hepatocyte nuclear factor (HNF)‐1α, HNF4, cytokeratin (CK)‐8, CK‐18, tyrosine amino transferase (TAT), and CYP2B6. Moreover, albumin was secreted, which suggests that MPCs from UCB possess multi‐differentiation potential and have the capacity to differentiate into functional cells of hepatic lineage in vitro .