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Effects of thymic polypeptides on the thymopoiesis of mouse embryonic stem cells
Author(s) -
Peng Yanwen,
Chen Zhenguang,
Yu Weihua,
Zhou Qifeng,
Xu Lin,
Mao Frank Fuxiang,
Huang Gang,
Zhang Xiuming,
Li Shug,
Lahn Bruce T.,
Xiang Andy Peng
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.07.011
Subject(s) - embryoid body , biology , embryonic stem cell , microbiology and biotechnology , cd8 , stem cell , t cell , t cell receptor , cd3 , cytotoxic t cell , haematopoiesis , immunology , immune system , adult stem cell , in vitro , gene , biochemistry
Abstract The thymus provides a unique cellular and hormonic microenvironment for the development of immunocompetent T cells. Thymic polypeptides have been widely used clinically for the treatment of tumors, infectious diseases and immune deficiency diseases. They have already shown the ability to stimulate the maturation of hematopoietic stem cells towards the CD3+CD4+ T cell lineage. However, their effects on the thymopoiesis of embryonic stem cells are still unexplored. In this paper, we compared the effects of three thymic polypeptides, thymopentin (TP5), thymosin alpha‐1 (Tα‐1) and thymopeptides on the in vitro thymopoiesis of mouse embryonic stem (ES) cells. Using the embryoid body induction system, we found that both Tα‐1 and thymopeptides effectively induced ES cells to differentiate sequentially into the CD3+ and CD4+/CD8+ T cells. These T cells had T cell receptor (TCR) Vβ gene rearrangement and most were TCRαβ T cells. We also found that the expression of the Notch receptor and its ligands Delta‐like‐1 and Delta‐like‐4 gradually increased during the induction. However, TP5 failed to induce the T cell differentiation of the ES cells. In summary, this is the first report to demonstrate that Tα‐1 can stimulate the T cell early stage differentiation from ES cells using the embryoid body protocol. These findings provide a powerful model for studying T cell development and may open new venues for the clinical application of Tα‐1.