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Identification of potential genes/proteins regulated by Tiam1 in colorectal cancer by microarray analysis and proteome analysis
Author(s) -
Liu Li,
Wang Shuang,
Zhang Qingling,
Ding Yanqing
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.07.004
Subject(s) - biology , colorectal cancer , metastasis , gene silencing , guanine nucleotide exchange factor , gene , annexin a2 , proteome , cancer research , microarray analysis techniques , cancer , gene expression , microbiology and biotechnology , genetics , cell , annexin , signal transduction
Tiam1 (T‐cell lymphoma invasion and metastasis‐inducing protein 1), a guanine nucleotide exchange factor that activates Rac, is a colorectal cancer metastasis‐related gene. In this study, we aimed to better understand the mechanism underlying Tiam1‐mediated metastasis. We applied gene microarray and proteome analysis and compared expression of genes and proteins in a stable Tiam1‐silencing colorectal cancer cell line and in a control cell line. Our analysis identified three genes, high‐mobility group box1 (HMGB1), annexin IV (ANXA4) and phosphoglycerate mutase 1 (PGAM1) that were associated with Tiam1. Analysis of these proteins, which may be directly or indirectly regulated by Tiam1, may provide insight into the role and mechanism of Tiam1 in colorectal cancer metastasis.