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Quality of embryonic bodies and seeding density effects on neural differentiation of mouse embryonic stem cells
Author(s) -
Zhou JunMei,
Xing FengYing,
Shi JianJun,
Fang ZhenFu,
Chen XueJin,
Chen Fang
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.04.025
Subject(s) - embryonic stem cell , neural stem cell , microbiology and biotechnology , seeding , neurosphere , neural development , biology , embryoid body , cellular differentiation , neural cell , embryo , stem cell , chemistry , anatomy , adult stem cell , cell , biochemistry , gene , agronomy
Mouse embryonic stem (ES) cells can be differentiated into neural lineage cells, but the differentiation efficiency remains low. This study revealed two important factors that influence the neural differentiation efficiency of mouse ES cells: the first is the quality of embryonic bodies (EBs); good quality of EBs consistently originated from a suspension culture of 1 × 10 5 ES cells/ml serum‐free chemically defined neural inducing medium and they exhibited a smooth round shape, with a dark central region surrounded by a light band. Such EBs are capable of attaining high neural differentiation efficiency. However, poor quality EBs originated from a suspension culture of 1 × 10 6 ES cells/ml serum‐free chemically defined neural inducing medium and exhibited an irregular shape or adhered to the bottom of the dish; they displayed low neural differentiation efficiency. The second factor is the seeding density of EBs: a low seeding density (5 EBs/cm 2 ) induced cells to differentiate into a more caudalized subtypes compared to the cells obtained from high seeding density (20 EBs/cm 2 ). These findings provided fresh insight into the neural induction of mouse ES cells.