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Cytoprotective activity of annphenone against oxidative stress‐induced apoptosis in V79‐4 lung fibroblast cells
Author(s) -
Shin Jung Sook,
Kang Kyoung Ah,
Kim Eun Sil,
Zhang Rui,
Piao Mei Jing,
Ko Dong Ok,
Wang Zhi Hong,
Maeng Young Hee,
Eun Su Yong,
Chae Sungwook,
Chung Ha Sook,
Hyun Jin Won
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.04.022
Subject(s) - oxidative stress , reactive oxygen species , apoptosis , lipid peroxidation , chemistry , fragmentation (computing) , tbars , antioxidant , dna fragmentation , programmed cell death , biochemistry , dna damage , microbiology and biotechnology , chinese hamster , biology , dna , ecology
We have elucidated the cytoprotective effect of annphenone (2,4‐dihyroxy‐6‐methoxy‐acetophenone 4‐ O ‐β‐ d ‐glucopyranoside) against oxidative stress‐induced apoptosis. Annphenone scavenged intracellular reactive oxygen species (ROS) and increased antioxidant enzyme activities. It thereby prevented lipid peroxidation and DNA damage, which was demonstrated by the inhibition of the formation of thiobarbituric acid reactive substance (TBARS), inhibition of the comet tail and decreased phospho‐H2A.X expression. Annphenone protected Chinese hamster lung fibroblast (V79‐4) cells from cell death via the inhibition of apoptosis induced by hydrogen peroxide (H 2 O 2 ), as shown by decreased apoptotic nuclear fragmentation, decreased sub‐G 1 cell population and inhibited mitochondrial membrane potential (Δ∍) loss. Taken together, these findings suggest that annphenone exhibits antioxidant properties by inhibiting ROS generation and thus protecting cells from H 2 O 2 ‐induced cell damage.