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Mitochondrial DNA from colorectal cancer cells promotes the malignant phenotype of NIH3T3 cells
Author(s) -
Li Yan,
Weibing Song,
Liu Hong,
Hongli Ji,
Zhuosheng Lai,
Yadong Wang,
Bing Xiao,
Daiming Fan
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.04.015
Subject(s) - carcinogenesis , mitochondrial dna , colorectal cancer , transfection , phenotype , biology , karyotype , cancer research , apoptosis , mitochondrion , cell , microbiology and biotechnology , cancer cell , chromosome , gene , cancer , cell culture , genetics
We investigated the potential role of mitochondrial DNA (mtDNA) in colorectal carcinogenesis by constructing a eukaryotic expression vector of the mitochondrial D‐loop gene from colorectal cancer cell SW480 and transfected NIH3T3 cells. The NIH3T3/SW480 cells exhibited a significantly increased growth rate and colony formation rate, and also had a decreased apoptotic rate. Polyploidy and aberrant chromosomes were detected in the NIH3T3/SW480 cells by chromosome karyotype analysis. Our results suggested that mtDNA from colorectal cancer cells promotes the malignant phenotype of NIH3T3 cells. Further study of the biological functions of NIH3T3/SW480 cells might be helpful in understanding the role of mtDNA in colorectal carcinogenesis.