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17β‐Estradiol affects proliferation and apoptosis of rat prostatic smooth muscle cells by modulating cell cycle transition and related proteins
Author(s) -
Luo Yi,
Waladali Wafi,
Li Shiwen,
Zheng Xinmin,
Hu Liquan,
Zheng Hang,
Hu Wanli,
Chen Chan
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.03.023
Subject(s) - apoptosis , cyclin d1 , hyperplasia , cell cycle , cell growth , cyclin , estrogen , microbiology and biotechnology , cell , chemistry , cyclin e , biology , transition (genetics) , cancer research , endocrinology , medicine , biochemistry , gene
Abundant evidence indicates that estrogens have an important role in the pathology of benign prostatic hyperplasia (BPH). To investigate the effect of 17β‐estradiol (E 2 ) on the proliferation and apoptosis of prostatic smooth muscle cells (PSMCs), rat PSMCs were obtained and exposed to gradient concentrations (0.1–100 nmol/l) of E 2 over varying amounts of time. The progression of cell cycle, cellular apoptosis, cyclin D1, Bcl‐2 and Bax proteins were detected. The data show that the effect of E 2 on rat PSMCs is bilateral: it promotes cell proliferation by enhancing the expression of cyclin D1, which accelerates G 1 to S phase transition; on the other hand, it induces apoptosis of the cells by up‐regulating the expression of Bax. We thus suggest that an increase in estrogen may exert a launching effect in the pathology of BPH.