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Effects of FGF2 and TGFβ 1 on the differentiation of human dental pulp stem cells in vitro
Author(s) -
He Huixia,
Yu Jinhua,
Liu Yuan,
Lu Shouchang,
Liu Hongchen,
Shi Junnan,
Jin Yan
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.03.013
Subject(s) - in vitro , dental pulp stem cells , transforming growth factor , pulp (tooth) , microbiology and biotechnology , stem cell , chemistry , biology , biochemistry , dentistry , medicine
Two crucial growth factors, FGF2 and TGFβ 1 , were investigated in this study to determine their inductive effects on the odontoblastic differentiation of human dental pulp stem cells (DPSCs) in vitro . DPSCs were isolated by immunomagnetic bead selection using the STRO‐1 antibody, and then co‐cultured respectively with FGF2, TGFβ 1 and FGF2 + TGFβ 1 . The results showed that FGF2 can exert a significant effect on the cell proliferation, while TGFβ 1 or FGF2 + TGFβ 1 can initiate an odontoblast‐like differentiation of DPSCs. Moreover, FGF2 can synergistically upregulate the effects of TGFβ 1 on the odontoblastic differentiation of DPSCs, as indicated by the increased alkaline phosphatase activity, the polarized cell appearance and secretary ultrastructural features, the formation of mineralized nodules and the gene/protein expression of dentin sialoprotein and dentin matrix protein‐1. Together, FGF2 acted primarily on the cell proliferation, while TGFβ 1 and FGF2 + TGFβ 1 mainly stimulated the odontoblastic differentiation of DPSCs. This study provides interesting progress in the odontoblastic differentiation of DPSCs induced by FGF2 and TGFβ 1 .